CIL:27153, unidentified plant. CIL. Dataset

NCBI Organism:unidentified plant; Biological process:reproduction

Licensing

This image is copyright protected. Any public or private use of this image is subject to prevailing copyright laws. Please contact the content provider of this image for permission requests.

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:27153^* Cite

Description

Z-stack through an unidentified pollen (mixed pollen grain w.m.; Carolina, commercial sample), with 200 image planes. See accompanying image in this group for a colorized maximum projection.

Technical Details

Pollen was imaged using an iMIC Andromeda Spinning Disk Confocal (TILL Photonics GmbH Objective: Olympus UPlanSApo 60X/NA1.35 CCD Camera: Imago QE (PCO). Excitation, 561nm Laser, with the following filters: Laser CleanUp, FF01-446/523/600/677 (Semrock); Dichroic, zt405/490/561/640rpc (Chroma, custom-made design); and emission filter, FF01-390/482/563/640 (Semrock).

Biological Sources

NCBI Organism Classification: unidentified plant

Biological Context

Biological Process: reproduction

Attribution

Names: Sebastian Rhode, Till Photonics

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL27153
Archival Resource Key (ARK): ark:/b7295/w9cil27153

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: spinning disk confocal microscopy
Parameters Imaged: fluorescence emission
Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	622px	0.1075µm
Y	455px	0.1075µm
Z	200px	0.2µm