CIL:26728, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

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NCBI Organism:Paramecium multimicronucleatum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:cytoplasm, early endosome, clathrin coat of endocytic vesicle, coated pit; Biological process:endosome organization, cytoplasm organization;

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:26728^* Cite

Description

Higher resolution of the area that shows the clathrin-coated cage near the peniculus and clathrin coated preendosomal and partially coated preendosomal vesicles. Early endosomes like the preendosomal vesicles have smooth E-fracture faces with few IMPs. TEM taken on 7/19/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 31,500X. Bar = 0.2µm. Adapted with permission from J. Cell Sci. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:12637) is also available for spatial analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: cytoplasm; early endosome; clathrin coat of endocytic vesicle; coated pit

Biological Context

Biological Process: endosome organization; cytoplasm organization

Attribution

Names: Richard Allen (University of Hawaii); C. Schroeder
Published: J. Cell Sci. 101:449-461, 1992
Pubmed: 1629255

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL26728
Archival Resource Key (ARK): ark:/b7295/w9cil26728

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: electron density
Parameters Imaged: elastic scattering of electrons; electron density; elevation
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: transmission electron microscopy (TEM)
Processing History: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data Qualifiers: processed data

Sample Preparation

Methods: freeze_fracture/freeze_etch
Methods: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2694px	——
Y	2136px	——