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NCBI Organism:Homo sapiens; Cell Components:, , spliceosomal complex; Biological process:, RNA metabolic process;
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:26588*  Cite 
Description

HeLa cells stably expressing BAC-encoded GFP-labeled snRNP protein U2A' and imaged witb a Leica SP5 confocal microscope using a 60x 1.4NA HCX Plan Apo objective lens. The U2A' is confined to the nucleoplasm and enriched in splicing factor compartments and Cajal Bodies (bright dots). See Fig 1A in Huranova et al. 2010 J Cell Biol 191:75-86.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
cervical epithelium
Cell Line
HeLa
Cellular Component
pre-mRNA
snRNP
spliceosomal complex
Biological Context
Biological Process
RNA splicing
RNA metabolic process
Attribution
Names
M. Huranova
I. Ivani
A. Benda
I. Poser
Y. Brody
M. Hof
Y. Shav-Tal
K.M. Neugebauer
D. Stanek
Published
M Huranova et al. 2010 J Cell Biol 191:75-86
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL26588
Archival Resource Key (ARK)
ark:/b7295/w9cil26588
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
Processing History
frame averaging
Data Qualifiers
processed data
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 512px ——
Y 512px ——