Image shows an immuno-FISH z-series of a mouse ES cell carrying a multi-copy insertion of alpha-globin BAC showing partial co-localization of lac operator FISH signal (green) with H3K9me3 immunostaining (red). DAPI staining is blue.
Formaldehyde fixed cells were imaged using an IMT-2 Olympus fluorescence microscope with 60x 1.4 NA objective lens. Z-series were recorded, and the slices processed by iterative constrained deconvolution. See also: P Sinclair et al. 2010 Dynamic plasticity of large scale chromatin structure revealed by self-assembly of engineered chromosome regions. J Cell Biol 109:761-776.
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 180px | 0.067µm |
| X | 233px | 0.067µm |
| Z | 19px | 0.2µm |
