CIL:26268, Xenopus laevis laevis, oocyte. CIL. Dataset

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Description

Movie shows spindle assembly around DNA-beads in a Xenopus laevis egg extract. DNA beads are autofluorescent, and spindle is formed from exogenous X-rhodamine-labeled tubulin. By the end of the movie a well-defined spindle has formed. Images were capured every 60 sec with a Hamamatsu Orca-ER cooled CCD camera using a Nikon TE2000-U stand and a 40x 1.3 NA objective lens in widefield mode. See Supplemental video 1 in Groen et al 2009, Mol Biol Cell 20:2766-2773.

Biological Sources

NCBI Organism Classification: Xenopus laevis laevis
Cell Type: oocyte
Cellular Component: Egg extract

Biological Context

Biological Process: spindle assembly involved in female meiosis

Attribution

Names: AC Groen; TJ Maresca; JC Gatlin; ED Salmon; TJ Michison
Published: Groen AC, Maresca TJ, Gatlin JC, Salmon ED, Mitchison TJ 2009 Mol Biol Cell 20:2766-2773
Pubmed: 19369413

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL26268
Archival Resource Key (ARK): ark:/b7295/w9cil26268

Grouping This image is part of a group.

Imaging

Image Type: charge coupled device (CCD)
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: X-Rhodamine; autofluorescence
Processing History: unprocessed raw data

Sample Preparation

Methods: unfixed tissue
Relation To Intact Cell: egg extract

Dimensions

Spatial Axis	Image Size	Pixel Size
X	198px	0.2µm
Y	198px	0.2µm

Time	60 seconds	60