EGFP-CP CALI experiments were performed by using a Spectra Physics Stabilite 2017 (Spectra Physics Laser Incorporated) argon ion laser (488 nm line, 2 W of beam power at the laser head) focused onto a 23.4 μm diameter spot (1/[e]2 diameter) through a 60x 1.45 N.A. PlanApo TIRF objective (Olympus). Irradiation was controlled with a fast Uniblitz (Vincent Associates) shutter. Laser power at the specimen plane dropped to 625 mW because of optical losses and was measured by placing the sensor of a laser power meter (Model FM with LS 10 head, Coherent Inc.) directly above the objective. Irradiation time was 100 ms, resulting in a 62.5-mJ dose of energy for the CALI experiment. Laser light was focused onto the specimen plane through a 488-nm laser filter set (Z488/10, HQ525/50, Z488RDC, Chroma Technology Corporation). Rat2 cells were plated onto 35-mm glass-bottomed dishes coated with 10 mg/ml fibronectin. Twenty minutes after plating, media was changed to DMEM without phenol red supplemented with 10% FBS, l-glutamine, and penicillin/streptomycin. DIC time-lapse imaging was performed on an Olympus IX81 inverted microscope equipped with a cooled 12-bit digital CCD camera (Sensicam QE, Cooke Corporation), and a heated chamber (Werner Instruments) with CO2 flow. All images were acquired with MetaMorph software (Version 6.0, Universal Imaging Corporation).