CIL:25698, Trichodina, Chlorella, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Trichodina, Chlorella, ; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:symbiont-containing vacuole, , oral apparatus, food vacuole, cytoplasm, macronucleus; Biological process:response to symbiont, oral apparatus organization, cytoplasm organization, , macronucleus organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:25698^* Cite

Description

A low power view of a median transverse section of a green Trichodina that lives on the surface of a freshwater snail collected in central Illinois. In this view the abundant endosymbiotic zoochlorellae and macronuclear extent are evident. A large food vacuole is present. TEM taken by G. Antipa in 1968 on a Hitachi HU11A operating at 75kV. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 65nm thickness.

Biological Sources

NCBI Organism Classification: Trichodina; Chlorella
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: symbiont-containing vacuole; zoochlorellae; oral apparatus; food vacuole; cytoplasm; macronucleus

Biological Context

Biological Process: response to symbiont; oral apparatus organization; cytoplasm organization; eukaryotic photosynthetic symbiont; macronucleus organization

Attribution

Names: Gregory Antipa (San Francisco State University)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL25698
Archival Resource Key (ARK): ark:/b7295/w9cil25698

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; unprocessed raw data
Data Qualifiers: raw, unprocessed data

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	5451px	——
Y	5374px	——