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NCBI Organism:Homo sapiens; Biological process:mitosis, ;
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Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:25694*  Cite 
Description

Time lapse series of cell growth and division in cultured hTERT-RPE1 cells (telomerase immortalized human retinal pigment epithelium) using phase contrast optics. A movie created from this time series is grouped with it in the Cell Image Library.

Technical Details

Cells were cultured on glass coverslips in 10% FBS, DMEM/F12 medium plus glutamine, HEPES, penicillin and streptomycin and examined with a Leica DMIRE2 microscope with Leica 40x HCX PL APO NA 1.25 objective lens. images were captured at 3 minute intervals with a Hamamatsu ORCA-ER2 CCD camera using Simple PCI 6.6.0.0 software.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
retinal pigment epithelium
Cell Line
hTERT-RPE1
Biological Context
Biological Process
mitosis
cell proliferation
Attribution
Names
Toshiyuki Hatano
Greenfield Sluder
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL25694
Archival Resource Key (ARK)
ark:/b7295/w9cil25694
Grouping This image is part of a group.
Imaging
Image Type
charge coupled device (CCD)
Image Mode
phase contrast microscopy
Parameters Imaged
elastic scattering of photons
Source of Contrast
differences in amount of elastic light scattering
Visualization Methods
visualization of contiguous regions
Processing History
unprocessed raw data
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 1344px 0.16µm
Y 1024px 0.16µm
Time 180 seconds 1922