CIL:25693, Homo sapiens, retinal pigment epithelium. CIL. Dataset

NCBI Organism:Homo sapiens; Biological process:mitosis, ;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:25693^* Cite

Description

Time series of cell growth and division in cultured hTERT-RPE1 cells (telomerase immortalized human retinal pigment epithelium) using phase contrast optics. A movie created with this series is grouped with it in the Cell Image Library.

Technical Details

Cells were cultured on glass coverslips in 10% FBS, DMEM/F12 medium plus glutamine, HEPES, penicillin and streptomycin and examined with a Leica DMIRE2 microscope with Leica 20x HC PL FLUOTAR NA 0.5 objective lens. images were captured at 3 minute intervals with a Hamamatsu ORCA-ER2 CCD camera using Simple PCI 6.6.0.0 software.

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: retinal pigment epithelium
Cell Line: hTERT-RPE1

Biological Context

Biological Process: mitosis; cell proliferation

Attribution

Names: Toshiyuki Hatano; Greenfield Sluder

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL25693
Archival Resource Key (ARK): ark:/b7295/w9cil25693

Grouping This image is part of a group.

Imaging

Image Type: charge coupled device (CCD)
Image Mode: phase contrast microscopy
Parameters Imaged: elastic scattering of photons
Source of Contrast: differences in amount of elastic light scattering
Visualization Methods: visualization of contiguous regions
Processing History: unprocessed raw data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1344px	0.322µm
Y	1024px	0.322µm

Time	180 seconds	486