Confocal fluorescent time-lapse imaging of bipolar spindle collapse induced by 1 uM taxol in a mitotic Drosophila S2 cell expressing GFP-tubulin. Images were recorded at 120 sec intervals using a spinning disc confocal microscope on a Nikon TE-200 stand with a 100x 1.4 NA Plan APO DIC objective lens and recorded with a Hamamatsu Orca-ER CCD. See Supplemental Video 6 in Maresca and Salmon, 2009 J Cell Biol 184:373-281.
Spatial Axis | Image Size | Pixel Size |
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X | 200px | 0.065µm |
Y | 200px | 0.065µm |
Time | 120 seconds | 40 |
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