CIL:24810, Xenopus laevis, keratocyte. CIL. Dataset

NCBI Organism:Xenopus laevis; Cell Components:lamellipodium, actin cytoskeleton, ; Biological process:, cellular localization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:24810^* Cite

Description

Localization of XAC XAC (Xenopus ADF/cofilin) to posterior regions of depolymerization-resistant actin brush. Fluorescence microscopy of lamellipodia of Xenopus keratocytes after latrunculin a (a binder of monomeric actin) treatment (0.25 μM for 10 min) and subsequent staining with XAC antibody and TRITC-phalloidin. Image corresponds to Figure 9b in J Cell Biol. 1999 May 31;145(5):1009-26.

Technical Details

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Biological Sources

NCBI Organism Classification: Xenopus laevis
Cell Type: keratocyte
Cellular Component: lamellipodium; actin cytoskeleton; ADF/cofilin

Biological Context

Biological Process: latrunculin a treatment; cellular localization

Attribution

Names: Tatyana M. Svitkina; Gary G. Borisy
Published: J Cell Biol. 1999 May 31;145(5):1009-26.
Pubmed: 10352018

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL24810
Archival Resource Key (ARK): ark:/b7295/w9cil24810

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: primary antibody plus labeled secondary antibody; Fluorescein (FITC); phalloidin; Tetramethyl rhodamine (TRITC)
Processing History: unprocessed raw data

Sample Preparation

Methods: glutaraldehyde fixed tissue; permeabilized tissue; phalloidin
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	499px	——
Y	963px	——