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NCBI Organism:Xenopus laevis; Cell Components:lamellipodium, actin cytoskeleton, , ; Biological process:cellular localization, actin filament-based process;
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:24807*  Cite 
Description

Localization of XAC (Xenopus ADF/cofilin) in Xenopus keratocytes done with immuno-EM. A higher mag view of a localized region of the cell is shown in CIL 24808. Image corresponds to Figure 8g from J Cell Biol. 1999 May 31;145(5):1009-26. A fluorescence image showing XAC and phalloidin labelin is available as CIL 24807.

Technical Details

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Biological Sources
NCBI Organism Classification
Xenopus laevis
Cell Type
keratocyte
Cellular Component
lamellipodium
actin cytoskeleton
lamella
ADF/Cofilin
Biological Context
Biological Process
cellular localization
actin filament-based process
Molecular Function
structural constituent of cytoskeleton
Attribution
Names
Tatyana M. Svitkina
Gary G. Borisy
Published
J Cell Biol. 1999 May 31;145(5):1009-26.
Pubmed
10352018
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL24807
Archival Resource Key (ARK)
ark:/b7295/w9cil24807
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
optical path length gradient
Source of Contrast
differences in deposition of metal shadow
Visualization Methods
shadowing and plating
platinum replica
immuno-gold
primary antibody plus labeled secondary antibody
Processing History
unprocessed raw data
Sample Preparation
Methods
glutaraldehyde fixed tissue
permeabilized tissue
polyethelyene glycol
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 2820px ——
Y 1512px ——