CIL:24801, Xenopus laevis, fibroblast. CIL. Dataset

NCBI Organism:Xenopus laevis; Cell Types:fibroblast Cell Components:lamellipodium, actin cytoskeleton, filopodium; Biological process:cellular localization, actin filament organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

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Description

Structural differentiation of actin network in lamellipodium. Electron micrograph of Xenopus fibroblasts after regular extraction in the presence of polyethelene glycol (PEG) and phalloidin. While the actin network in the front zone of these cels remains dense, the actin network at the lamellipodial rear disassembled in the course of unprotected extraction (CIL 24800). Image corresponds to Figure 6c from J Cell Biol. 1999 May 31;145(5):1009-26.

Technical Details

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Biological Sources

NCBI Organism Classification: Xenopus laevis
Cell Type: fibroblast
Cellular Component: lamellipodium; actin cytoskeleton; filopodium

Biological Context

Biological Process: cellular localization; actin filament organization
Molecular Function: structural constituent of cytoskeleton

Attribution

Names: Tatyana M. Svitkina; Gary G. Borisy
Published: J Cell Biol. 1999 May 31;145(5):1009-26.
Pubmed: 10352018

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL24801
Archival Resource Key (ARK): ark:/b7295/w9cil24801

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: optical path length gradient
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: shadowing and plating; platinum replica
Processing History: unprocessed raw data

Sample Preparation

Methods: permeabilized tissue; glutaraldehyde fixed tissue; phalloidin; polyethelene glycol
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4380px	——
Y	3360px	——