CIL:24800, Xenopus laevis, keratocyte. CIL. Dataset

NCBI Organism:Xenopus laevis; Cell Components:lamellipodium, actin cytoskeleton; Biological process:actin filament organization

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:24800^* Cite

Description

Structural differentiation of actin network in lamellipodium. Electron micrograph of Xenopus keratocyte after unprotected extraction without PEG and phalloidin. Actin network at lamellipodial rear disassembled in the course of unprotected extraction, whereas front zone remained as dense as in control cells (CIL 24801). Image corresponds to Figure 6b from J Cell Biol. 1999 May 31;145(5):1009-26.

Technical Details

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Biological Sources

NCBI Organism Classification: Xenopus laevis
Cell Type: keratocyte
Cellular Component: lamellipodium; actin cytoskeleton

Biological Context

Biological Process: actin filament organization
Molecular Function: structural constituent of cytoskeleton

Attribution

Names: Tatyana M. Svitkina; Gary G. Borisy
Published: J Cell Biol. 1999 May 31;145(5):1009-26.
Pubmed: 10352018

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL24800
Archival Resource Key (ARK): ark:/b7295/w9cil24800

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: optical path length gradient
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: shadowing and plating; platinum replica
Processing History: unprocessed raw data

Sample Preparation

Methods: permeabilized tissue; glutaraldehyde fixed tissue
Relation To Intact Cell: dispersed cell in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4356px	——
Y	3229px	——