CIL:24796, fibroblast. CIL. Dataset

Cell Types:fibroblast Cell Components:lamellipodium, actin cytoskeleton; Biological process:cellular macromolecule localization

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:24796^* Cite

Description

Localization of Arp2/3 complex in a fibroblast lamellioidia. Staining with p21 antibody (green) and TRIT-phalloidin (red) shows Arp2/3 complex highly enriched in lamellipodia. CIL 24796 shows an immuno EM image of Arp2/3 localization in a fibroblast. CIL 24794 and 24795 show corresponding images in Xenopus keratocytes. Image corresponds to Figure 3e-g from J Cell Biol. 1999 May 31;145(5):1009-26.

Technical Details

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Biological Sources