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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:24795*  Cite 
Description

Localization of Arp2/3 complex in the lamellioidia of a Xenopus keratocytes. Staining with p21 antibody and 10 nm gold secondary antibody. CIL 24794 shows a fluorescence microsopy image of Arp2/3 localization in a Xenopus keratocyte. CIL 24796 and 24797 show corresponding images in fibroblasts. Image corresponds to Figure 3d from J Cell Biol. 1999 May 31;145(5):1009-26.

Technical Details

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Biological Sources
NCBI Organism Classification
Xenopus laevis
Cell Type
keratocyte
Cellular Component
lamellipodium
actin cytoskeleton
Biological Context
Biological Process
cellular macromolecule localization
Attribution
Names
Tatyana M. Svitkina
Gary G. Borisy
Published
J Cell Biol. 1999 May 31;145(5):1009-26.
Pubmed
10352018
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL24795
Archival Resource Key (ARK)
ark:/b7295/w9cil24795
Grouping This image is part of a group.
Sample Preparation
Methods
glutaraldehyde fixed tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 1022px 4.5nm
Y 1410px 4.5nm