CIL:24794, Xenopus laevis, keratocyte. CIL. Dataset

NCBI Organism:Xenopus laevis; Cell Components:lamellipodium, actin cytoskeleton; Biological process:cellular macromolecule localization

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:24794^* Cite

Description

Localization of Arp2/3 complex in the lamellioidia of a Xenopus keratocytes. Staining with p21 antibody (green) and TRIT-phalloidin (red) shows Arp2/3 complex highly enriched in lamellipodia. CIL 24795 shows an immuno EM image of Arp2/3 localization in a Xenopus keratocyte. CIL 24796 and 24797 show corresponding images in fibroblasts. Image corresponds to Figure 3a-c from J Cell Biol. 1999 May 31;145(5):1009-26.

Technical Details

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Biological Sources