CIL:24786, Xenopus laevis, keratocyte. CIL. Dataset

NCBI Organism:Xenopus laevis; Cell Components:lamellipodium, actin cytoskeleton; Biological process:, actin filament-based process;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:24786^* Cite

Description

Multiple branching of actin filaments in lamellipodia of Xenopus keratocytes. This image shows an overview of the leading edge, and CIL 24787 shows enlargements of local regions of this platinum replica. CIL 24788 shows a similar overview for the lamellipodia of a vertebrate fibroblasts. Image corresponds to Figure 1a from J Cell Biol. 1999 May 31;145(5):1009-26.

Technical Details

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Biological Sources

NCBI Organism Classification: Xenopus laevis
Cell Type: keratocyte
Cellular Component: lamellipodium; actin cytoskeleton

Biological Context

Biological Process: branching of actin filaments; actin filament-based process

Attribution

Names: Tatyana M. Svitkina; Gary G. Borisy
Published: J Cell Biol. 1999 May 31;145(5):1009-26.
Pubmed: 10352018

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL24786
Archival Resource Key (ARK): ark:/b7295/w9cil24786

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: optical path length gradient
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: shadowing and plating; platinum replica
Processing History: unprocessed raw data

Sample Preparation

Methods: permeabilized tissue; glutaraldehyde fixed tissue; phalloidin; polyethylene glycol
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3286px	——
Y	3268px	——