CIL:24783, Xenopus laevis, fibroblast. CIL. Dataset

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NCBI Organism:Xenopus laevis; Cell Types:fibroblast Cell Components:lamellipodium, actin cytoskeleton, ; Biological process:cellular localization

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:24783^* Cite

Description

Localization of XAC (Xenopus ADF/cofilin) in Xenopus fibroblasts. Fluorescence microscopy of a cell fragment double stained with XAC antibody (green) and TRITC-phalloidin (red). XAC is distributed throughout the entire lamellipodium. Image corresponds to Figure 10a-c from J Cell Biol. 1999 May 31;145(5):1009-26.

Technical Details

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Biological Sources

NCBI Organism Classification: Xenopus laevis
Cell Type: fibroblast
Cellular Component: lamellipodium; actin cytoskeleton; Xenopus ADF/cofilin

Biological Context

Biological Process: cellular localization

Attribution

Names: Tatyana M. Svitkina; Gary G. Borisy
Published: J Cell Biol. 1999 May 31;145(5):1009-26.
Pubmed: 10352018

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL24783
Archival Resource Key (ARK): ark:/b7295/w9cil24783

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein; distribution of epitope
Visualization Methods: Fluorescein (FITC); Tetramethyl rhodamine (TRITC); phalloidin; primary antibody plus labeled secondary antibody
Processing History: unprocessed raw data

Sample Preparation

Methods: glutaraldehyde fixed tissue; permeabilized tissue; polyethylene glycol
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2339px	——
Y	572px	——