CIL:24733, Homo sapiens, neuroblastoma. CIL. Dataset

NCBI Organism:Homo sapiens; Cell Components:nucleolus, chromatin; Biological process:ribosome biogenesis, nucleus organization, RNA metabolic process, nucleolus organization, chromatin organization;

Licensing

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CIL:24733^* Cite

Description

Thin section showing nucleolus of a Human neuroblastoma SK-N-SH cell imaged with a FEI Tecnai 20 energy filtered electron microscope at 200 KV and a 20 eV energy window. Overlay of net phosphorus distribution (yellow) and nitrogen minus phosphorus (blue). PML, PML body; FC, fibrillar component of nucleolus; DFC, dense fibrillar component of nucleolus; GC, granular component of nucleolus.

Technical Details

Net phosphorus distribution computed from images taken with energy losses of 155 eV and 155 eV bracketing the phosphorus 'edge', and corresponding nitrogen distribution computed from images taken at 385 eV and 415 eV. See Fig 7D in Politz et al., 2005 Mol Biol Cell 16:3401-3410.

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: neuroblastoma
Cell Line: SK-N-SH
Cellular Component: nucleolus; chromatin

Biological Context

Biological Process: ribosome biogenesis; nucleus organization; RNA metabolic process; nucleolus organization; chromatin organization

Attribution

Names: Joan Politz
Published: Politz et al., 2005 Mol Biol Cell 16:3401-3410
Pubmed: 15857956
Link: Molecular Biology of the Cell
OTHER: Ilvin Polena; Ian Trask; David Bazett-Jones; Thoru Pederson

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL24733
Archival Resource Key (ARK): ark:/b7295/w9cil24733

Grouping This image is part of a group.

Imaging

Image Type: charge coupled device (CCD)
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: inelastic scattering
Visualization Methods: Electron energy loss
Processing History: multipanel montage; false color; computed image
Data Qualifiers: processed data

Sample Preparation

Methods: formaldehyde fixed tissue; glutaraldehyde fixed tissue; tissue in plastic embedment
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	435px	——
X	432px	——