This ghoulish-looking Schwann cell was present in a primary culture of the dorsal root ganglion of mouse that was transfected with a mutant peripherin transgene. The intermediate filament network (vimentin antibody labeling in green) is collapsed around the nucleus 48 hr after transfection, and is correlated with a corresponding peri-nuclear clustering of the mitochondria (labeled with Mitotracker). Mitotracker CMX Ros was added to the culture medium for 30 mins, then rinsed out for 45 minutes in culture before fixing the cells with methanol. Microscope - Zeiss Axioplan I Illumination - Mercury arc lamp Objective - 100X oil/1.3 NA Intermediate lens between obj and camera - 0.63X Camera - Axiocam MRM CCD Filters: 1. Ex 485/20, Em 515-565 BP, dichroic 510 2. Ex 560/40, Em 630/60, dichroic 595