Mitochondrial distribution (visualized with Mitotracker CMXRos) was largely unaffected by partial fragmentation of the intermediate filament cytoskeleton, visualized here with vimentin antibody, after expression of a mutant form of the peripherin gene in a primary fibroblast. This primary culture derived from the mouse dorsal root ganglion was methanol-fixed at -20 degrees. Microscope - Zeiss Axioplan I Illumination - Mercury arc lamp Objective - 63X oil/1.4 NA Intermediate lens between obj and camera - 0.63X Camera - Axiocam MRM CCD Filters: 1. Ex 485/20 BP, Em 515-565 BP 2. Ex 560/40, Em 630/6