CIL:23610, Saccharomyces cerevisiae S288c. CIL. Dataset

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:23610^* Cite

Description

Free microtubule sliding in cnm67delta mutant cells expressing GFP-Tubulin (GFP-TUB1; left panel, green in right panel merge) and RFP-Spc97 (a component of the gamma-tubulin complex, marks the minus end; middle panel, red in right panel merge). Movie plays at 50x real time and is movie S9 in Proc Natl Acad Sci. 2009. 106: 5147-5152.

Technical Details

S. cerevisiae (MATa SPC97-tdimer2::KanR cnm67delta::TRP1 ura3-52 lys2-801 leu2-delta1::GFP-TUB1::LEU2 his3-delta200 trp1-delta63) were grown to midlog phase and mounted on agarose pads for microscopy. Images were collected on an Olympus IX70 inverted fluorescence microscope with a 100 N.A. 1.35 oil objective lens and a CoolSNAP HQ camera (Roper Scientific), using QED software (Media Cybernetics). Timelapse images were captured at 5-s intervals.

Biological Sources

NCBI Organism Classification: Saccharomyces cerevisiae S288c
Cell Line: cnm67delta
Cellular Component: spindle pole body; cytoplasmic microtubule; outer plaque of spindle pole body; gamma-tubulin small complex, spindle pole body; inner plaque of spindle pole body

Biological Context

Biological Process: microtubule-based movement; mitotic spindle organization; microtubule cytoskeleton organization; microtubule nucleation; cell division
Molecular Function: GTPase activity; structural constituent of cytoskeleton

Attribution

Names: Jeffrey K. Moore; David Sept; John A. Cooper
Published: Proc Natl Acad Sci. 2009. 106: 5147-5152.
Pubmed: 19279216

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL23610
Archival Resource Key (ARK): ark:/b7295/w9cil23610

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: widefield illumination; fluorescence microscopy; time lapse microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: EGFP; DsRed2FP
Data Qualifiers: raw, unprocessed data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	279px	——
Y	74px	——

Time	5 seconds	61