CIL:23609, Saccharomyces cerevisiae S288c. CIL. Dataset

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:23609^* Cite

Description

Microtubule detachment from the spindle pole body in kar9delta nip100deltaCAP-gly double mutant cells arrested in S phase. Movie plays at 20x real time and is movie S8 in Proc Natl Acad Sci. 2009. 106: 5147-5152.

Technical Details

S. cerevisiae (MATa NIP100deltaCAP-Gly DYN1-3GFP::TRP1 kar9delta::hygB ura3-52 lys2-801 leu2-delta1::GFP-TUB1::LEU2 his3-delta200 trp1-delta63) expressing GFP-tubulin (GFP-TUB1) and dynein heavy chain tagged with 3GFP (DYN1-3GFP) were arrested in hydroxyurea and mounted on agarose pads for microscopy. Images were captured on an Olympus Bmax-60F microscope equipped with a 1.35NA 100× UPlanApo objective, spinning disc Confocal Scanner Unit (CSU10), Picarro Cyan laser (488 nm), and a Stanford Photonics XR-Mega10 ICCD camera, by using QED software (Media Cybernetics). Image analysis was performed by using ImageJ. Timelapse images were captured at 10-s intervals for 15 min and each image in the movie represents a composite of 9 planes separated by 500 nm.

Biological Sources

NCBI Organism Classification: Saccharomyces cerevisiae S288c
Cell Line: NIP100deltaCAP-Gly, kar9delta
Cellular Component: spindle pole body; cytoplasmic microtubule; microtubule; dynein complex; dynactin complex; spindle pole; cell cortex

Biological Context

Biological Process: microtubule-based movement; karyogamy; nuclear migration along microtubule; establishment of mitotic spindle orientation; mitotic spindle elongation
Molecular Function: GTPase activity; structural constituent of cytoskeleton; microtubule binding; motor activity; ATPase activity; ATP binding; microtubule motor activity

Attribution

Names: Jeffrey K. Moore; David Sept; John A. Cooper
Published: Proc Natl Acad Sci. 2009. 106: 5147-5152.
Pubmed: 19279216

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL23609
Archival Resource Key (ARK): ark:/b7295/w9cil23609

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: spinning disk confocal microscopy; time lapse microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: EGFP
Processing History: each frame is a composite of 9 planes
Data Qualifiers: processed data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	73px	——
Y	136px	——

Time	10 seconds	25