CIL:21666, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Paramecium multimicronucleatum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:cytoplasm, clathrin-coated endocytic vesicle membrane, endosome; Biological process:cytoplasm organization, endosome organization, clathrin coat assembly;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:21666^* Cite

Description

A clump of at least seven small clathrin cages that are probably connected to an early endosome (below the fracture plane). The morphology of these cages shows a tighter packing than the larger clathrin cages at the parasomal sacs. TEM taken on 7/29/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 31,500X. Bar = 0.2µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:12640) is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: cytoplasm; clathrin-coated endocytic vesicle membrane; endosome

Biological Context

Biological Process: cytoplasm organization; endosome organization; clathrin coat assembly

Attribution

Names: Richard Allen (University of Hawaii); C. Schroeder

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL21666
Archival Resource Key (ARK): ark:/b7295/w9cil21666

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: electron density
Parameters Imaged: elastic scattering of electrons; electron density; elevation
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: transmission electron microscopy (TEM)
Processing History: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data Qualifiers: processed data

Sample Preparation

Methods: freeze_fracture/freeze_etch
Methods: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2129px	——
Y	2352px	——