CIL:21615, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Paramecium multimicronucleatum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:cell cortex, clathrin coat, , microtubule basal body; Biological process:cortical cytoskeleton organization, endosome organization, cytoplasm organization, microtubule basal body organization;

Licensing

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:21615^* Cite

Description

A view of an early endosome with at least two coated evaginations showing, lies next to the proximal end of a fractured basal body. Other clathrin cages may indicate other evaginations from the same early endosome or from another nearby early endosome. TEM taken on 6/6/91 by R. Allen with Zeiss 10A operating at 80kV. Neg. 31,500X. Bar = 0.2µm. Published in J. Cell Sci. 101:449-461, 1992. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:12639) is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: cell cortex; clathrin coat; endosome; microtubule basal body

Biological Context

Biological Process: cortical cytoskeleton organization; endosome organization; cytoplasm organization; microtubule basal body organization

Attribution

Names: Richard Allen (University of Hawaii)
Published: J. Cell Sci. 101:449-461, 1992
Pubmed: 1629255

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL21615
Archival Resource Key (ARK): ark:/b7295/w9cil21615

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: electron density
Parameters Imaged: elastic scattering of electrons; electron density; elevation
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: transmission electron microscopy (TEM)
Processing History: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data Qualifiers: processed data

Sample Preparation

Methods: freeze_fracture/freeze_etch
Methods: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2754px	——
Y	2165px	——