Fluorescence images of control and Si-RNA transfected HeLa cells treated with nocodazole and allowed to recover. Mitotic cells were stained for gamma-tubulin (top row, green)to label centrosomes or NuMA (center row, red). Merges images inclde DAPI staining of DNA (blue). Multiple NuMA signals are not due to centrosome amplification. Cells were observed with an Olympus IX70 microscope and recorded with a Magnaview CCD camera. See Fig 4C in Kong et al., 2009, Mol Biol Cell 20:1289-1301.