CIL:1454, Oryctolagus cuniculus, skeletal muscle cell. CIL. Dataset

NCBI Organism:Oryctolagus cuniculus; Cell Types:skeletal muscle cell Cell Components:cytoskeleton Biological process:skeletal muscle contraction

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:1454^* Cite

Description

Rabbit psoas skeletal muscle fibers were held at constant length and rigor induced by immersion in glycerol. The fibers were quick-frozen by contact with a liquid rprs_7544196.tifhelium- cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 24 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev. Images were recorded on Kodak 4489 film at 75,000x magnification. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner. Stereo pair with CIL:2874.

Biological Sources

NCBI Organism Classification: Oryctolagus cuniculus
Cell Type: skeletal muscle cell
Cellular Component: cytoskeleton

Biological Context

Biological Process: skeletal muscle contraction
Molecular Function: ATPase activity, coupled to movement of substances

Attribution

Names: Ip, Wallace

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL1454
Archival Resource Key (ARK): ark:/b7295/w9cil1454

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: transmission electron microscopy (TEM); wide-field detection; widefield illumination; illumination by electrons; detection of electrons
Parameters Imaged: electron density; elevation
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: shadowing and plating
Processing History: unprocessed raw data

Sample Preparation

Methods: glutaraldehyde fixed tissue; freeze_fracture/freeze_etch
Relation To Intact Cell: isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4183px	0.00027µm
Y	2797px	0.00027µm