Representative image indicative of autophagic activity of C. elegans (WT; N2 Bristol isolate) embryo expressing a full-length plgg-1DsRED::LGG-1 fusion fed with UV-killed Escherichia coli without additional 0.2mM spermidine. Epifluorescence microscopy was performed with a microscope (AxioImager Z2; Carl Zeiss, Inc.) using a Plan Neofluar 40× objective with a 0.75 NA and a 63× Plan Neofluar objective with an NA of 1.25 in oil at RT using a 540 ± 15–nm band-pass excitation filter and a 575-nm long-pass emission filter. Images were taken with a camera (AxioCam MRc5; Carl Zeiss, Inc.) with Axiovision software (Carl Zeiss, Inc.) without further processing. Image: Figure 3A, top left panel, in Morselli et al. J Cell Biol 192: 615-629