Saccharomyces cerevisiae (WT BY4741-MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0) undergoing chronological aging on small synthetic 2% glucose media (SMD) with supplementation of 4 mM spermidine. Spermidine was added to stationary cultures at day 1 of the aging experiments. Image represents EGFP-Atg8p localization, visualized by fluorescence microscopy. Yeast cells undergoing autophagy exhibit a prominent vacuolar localization of EGFP-Atg8p. Epifluorescence image was captured using a widefield microscope (Axioskop; Carl Zeiss, Inc) fitted with a Plan Neofluar 63× 1.25 NA oil immersion objective. Image acquired with a camera (SPOT 9.0 Monochrome 6; Diagnostic Instruments, Inc) using Metamorph software (Universal Imaging Corp) and processed with IrfanView and Photoshop (CS2; Adobe) software. Specifically, picture processing involved coloring and cropping of representative areas and was performed with IrfanView. In addition, linear adjustments of contrast and brightness were applied with Photoshop (using equal adjustment parameters for all pictures); no explicit γ correction was used. Image: Figure 2A, WT/Spd/EGFP-Atg8p panel, in Morselli et al. J Cell Biol 192: 615-629