CIL:13896, Saccharomyces cerevisiae. CIL. Dataset

NCBI Organism:Saccharomyces cerevisiae; Cell Components:spindle pole body Biological process:mitotic cell cycle spindle orientation checkpoint, protein phosphorylation, regulation of exit from mitosis, small GTPase mediated signal transduction, mitosis, cell division;

Licensing

Attribution Non-Commercial Share Alike:This image is licensed under a Creative Commons Attribution, Non-Commercial Share Alike License. View License Deed | View Legal Code

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13896^* Cite

Description

DIC image of cells constitutively expressing Tem1 localized to both spindle pole bodies (SPBs) and expressing Kin4 from the GAL1-10 promoter growing in medium with galactose. Constitutive loading of Tem1 to SPBs allowed cells to progress through the cell cycle despite Kin4 overexpression. Compare to control image CIL# 13897. Image is Fig 5C bottom right panel in J Cell Biol. (2011) 192: 599-614. Other images in Fig 5 include CIL# 13895, 13894, 13897, 13896.

Technical Details

Cells (MATa tem1::KanMX ura3::eGFP-CNM67–TEM1::URA3 kin4::TRP1-pGAL1-10-KIN4) were grown in rich medium containing 2% galactose/ 2% raffinose. Imaging was performed at 25C using a Leica DM6000 microscope equipped with a 100x/1.40 NA oil immersion objective lens, and a digital CCD camera (DFC350, Leica). Pictures were processed with LAS AF (leica) and ImageJ software.

Biological Sources

NCBI Organism Classification: Saccharomyces cerevisiae
Cell Line: W303
Cellular Component: spindle pole body

Biological Context

Biological Process: mitotic cell cycle spindle orientation checkpoint; protein phosphorylation; regulation of exit from mitosis; small GTPase mediated signal transduction; mitosis; cell division
Molecular Function: ATP binding; protein serine/threonine kinase activity; GTPase activity; nucleotide binding; protein binding

Attribution

Names: Mauricio Valerio-Santiago; Fernando Monje-Casas
Published: J Cell Biol. (2011) 192: 599-614.
Pubmed: 21321099
Link: http://jcb-dataviewer.rupress.org/jcb/browse/...

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL13896
Archival Resource Key (ARK): ark:/b7295/w9cil13896

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: differential interference contrast microscopy
Parameters Imaged: optical path length gradient
Source of Contrast: boundaries between regions with different refractive index
Processing History: unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	621px	0.0644µm
Y	621px	0.0644µm