CIL:13894, Saccharomyces cerevisiae. CIL. Dataset

NCBI Organism:Saccharomyces cerevisiae; Cell Components:spindle pole body Biological process:mitotic cell cycle spindle orientation checkpoint, protein phosphorylation;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13894^* Cite

Description

DIC image of cells expressing Kin4 from the GAL1-10 promoter and growing in medium with galactose. Overexpression of Kin4 arrests cells in anaphase. Compare with control image CIL# 13895. Image is Fig 5C top right panel in J Cell Biol. (2011) 192: 599-614. Other images in Fig 5 include CIL# 13895, 13894, 13897, 13896.

Technical Details

Cells (MATa kin4::TRP1-pGAL1-10-KIN4) were grown in rich media containing 2% galactose/2% raffinose. Imaging was performed at 25C using a Leica DM6000 microscope equipped with a 100x/1.40 NA oil immersion objective lens, and a digital CCD camera (DFC350, Leica). Pictures were processed with LAS AF (leica) and ImageJ software.

Biological Sources

NCBI Organism Classification: Saccharomyces cerevisiae
Cell Line: W303
Cellular Component: spindle pole body

Biological Context

Biological Process: mitotic cell cycle spindle orientation checkpoint; protein phosphorylation
Molecular Function: ATP binding; protein serine/threonine kinase activity

Attribution

Names: Mauricio Valerio-Santiago; Fernando Monje-Casas
Published: J Cell Biol. (2011) 192: 599-614.
Pubmed: 21321099
Link: http://jcb-dataviewer.rupress.org/jcb/browse/...

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL13894
Archival Resource Key (ARK): ark:/b7295/w9cil13894

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: differential interference contrast microscopy
Parameters Imaged: optical path length gradient
Source of Contrast: boundaries between regions with different refractive index
Processing History: unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	621px	0.0644µm
Y	621px	0.0644µm