CIL:13727, Mus musculus, fibroblast. CIL. Dataset

NCBI Organism:Mus musculus; Cell Types:fibroblast Cell Components:mitochondrion, cytosol, nucleus, mitochondrial inner membrane, mitochondrial outer membrane; Biological process:mitochondrion degradation, protein ubiquitination involved in ubiquitin-dependent protein catabolic process, response to stress, protein phosphorylation, intracellular protein kinase cascade, protein K48-linked ubiquitination, protein K63-linked ubiquitination;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13727^* Cite

Description

Upon mitochondrial uncoupling, wild-type PINK1-YFP (green) and mCherry-Parkin (red) are localized to mitochondria in PINK1 knock-out mouse embryonic fibroblasts (PINK1 KO MEFs). Transfected PINK1 KO MEFs were treated with the mitochondrial depolarizing agent CCCP (carbonyl cyanide-m-chlorophenyl hydrazone) (10µM) for 3 hours. Live cell imaging was performed on an LSM510 Meta (Carl Zeiss, Inc) with a 63x 1.4 NA oil differential interference contrast Plan Apo objective. Image contrast and brightness were adjusted in the LSM image browser (Zeiss). This image corresponds to Supplemental Figure S1c, bottom left row of J Cell Biol, 191: 933-942, 2010. Images in Supplemental Figure S1c include CIL#s 13725, 13726, 13727, 13728.

Biological Sources

NCBI Organism Classification: Mus musculus
Cell Type: fibroblast
Cellular Component: mitochondrion; cytosol; nucleus; mitochondrial inner membrane; mitochondrial outer membrane

Biological Context

Biological Process: mitochondrion degradation; protein ubiquitination involved in ubiquitin-dependent protein catabolic process; response to stress; protein phosphorylation; intracellular protein kinase cascade; protein K48-linked ubiquitination; protein K63-linked ubiquitination
Molecular Function: ubiquitin-protein ligase activity; zinc ion binding; protein binding; protein serine/threonine kinase activity; ATP binding; magnesium ion binding; ubiquitin protein ligase binding; calcium-dependent protein kinase activity; C3HC4-type RING finger domain binding; oxidative phosphorylation uncoupler activity

Attribution

Names: Seok Min Jin; Michael Lazarou; Chunxin Wang; Lesley A. Kane; Derek P. Narendra; Richard J. Youle
Published: J Cell Biol, 191: 933-942, 2010.
Pubmed: 21115803
Link: http://jcb-dataviewer.rupress.org/jcb/browse/...

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL13727
Archival Resource Key (ARK): ark:/b7295/w9cil13727

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: single-spot confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: EYFP; mCherryFP
Processing History: brightness and contrast adjusted
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	512px	0.1395µm
Y	512px	0.1395µm