CIL:13592, Mus musculus, myoblast. CIL. Dataset

NCBI Organism:Mus musculus; Cell Types:myoblast Cell Components:nucleus, muscle myosin complex; Biological process:myoblast fusion

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13592^* Cite

Description

The C2C12 cell line, a mouse myoblast line, was used here to study the regulatory factors in myogenic differentiation. After cultured in differentiation medium for 3 days, these cells differentiated into myotubes (Green) containing multiple nuclei (Blue). The C2C12 cells stably expressing FLAG-tagged rapamycin-resistant, kinase-inactive (RR/KI) mTOR were induced to differentiation in the presence of 50nM rapamycin for 3 days. The C2C12 cells differentiated, but arrested at the nascent myotube stage. This image is the control of different treatments in Figure 9B from JCB 189: 1157-1169, 2010. See also CIL: 13593, 13594, 13601.

Technical Details

The image was taken by leica DMI 4000B microscope equipped with a QImaging RETIGA Exi camera and Leica 10X/0.22 lens. Myosin heavy chain (MHC) expressed in myotubes was labeled by MF-20 mouse antibody followed by FITC-anti-mouse IgG secondary antibody. Nuclei were stained by DAPI. MHC and nuclei were pseudocolored in green and blue, respectively.

Biological Sources

NCBI Organism Classification: Mus musculus
Cell Type: myoblast
Cell Line: C2C12
Cellular Component: nucleus; muscle myosin complex

Biological Context

Biological Process: myoblast fusion
Molecular Function: mTOR inhibition

Attribution

Names: Yuting Sun; Yejing Ge; Jenny Drnevich; Yong Zhao; Mark Band; Jie Chen
Published: JCB 189: 1157-1169, 2010
Pubmed: 20566686

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL13592
Archival Resource Key (ARK): ark:/b7295/w9cil13592

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: compartmentalization of stain or label; distribution of epitope
Visualization Methods: Fluorescein (FITC); 4',6-diamidino-2-phenylindole (DAPI); primary antibody plus labeled secondary antibody
Processing History: brightness and contrast adjusted
Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: formaldehyde fixed tissue; permeabilized tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1392px	0.645µm
Y	1040px	0.645µm