CIL:13589, Mus musculus, myoblast. CIL. Dataset

NCBI Organism:Mus musculus; Cell Types:myoblast Cell Components:nucleus, muscle myosin complex; Biological process:myoblast fusion

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13589^* Cite

Description

The C2C12 cell line, a mouse myoblast line, was used here to study the regulatory factors in myogenic differentiation. After cultured in differentiation medium for 3 days, these cells differentiated into myotubes (Green) containing multiple nuclei (Blue). The myoblasts in this image were untreated during differentiation. This image is the control of different treatments in Figure 9A from JCB 189: 1157-1169, 2010. See also CIL: 13590, 13591.

Technical Details

The image was taken by leica DMI 4000B microscope equipped with a QImaging RETIGA Exi camera and Leica 10X/0.22 lens. Myosin heavy chain (MHC) expressed in myotubes was labeled by MF-20 mouse antibody followed by FITC-anti-mouse IgG secondary antibody. Nuclei were stained by DAPI. MHC and nuclei were pseudocolored in green and blue, respectively.

Biological Sources

NCBI Organism Classification: Mus musculus
Cell Type: myoblast
Cell Line: C2C12
Cellular Component: nucleus; muscle myosin complex

Biological Context

Biological Process: myoblast fusion

Attribution

Names: Yuting Sun; Yejing Ge; Jenny Drnevich; Yong Zhao; Mark Band; Jie Chen
Published: JCB 189: 1157-1169, 2010
Pubmed: 20566686

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL13589
Archival Resource Key (ARK): ark:/b7295/w9cil13589

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: compartmentalization of stain or label; distribution of epitope
Visualization Methods: Fluorescein (FITC); 4',6-diamidino-2-phenylindole (DAPI); primary antibody plus labeled secondary antibody
Processing History: brightness and contrast adjusted
Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: formaldehyde fixed tissue; permeabilized tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1392px	0.645µm
Y	1040px	0.645µm