The C2C12 cell line, a mouse myoblast line, was used here to study the regulatory factors in myogenic differentiation. After cultured in differentiation medium for 3 days, these cells differentiated into myotubes (Green) containing multiple nuclei (Blue). The myoblasts in this image were transfected with the locked nucleic acid (LNA) anti-miR-1. During the last 2 days of differentiation, cells were treated with 0.2ug/ml recombinant follistatin. The effect of miR-1 was rescued by follistatin. This image is the control of different treatments in Figure 6B from JCB 189: 1157-1169, 2010. See also CIL: 13585, 13586, 13587.