FISH detection of FOLS2 (FRA2; fluorescein, green) and CSF1R (rhodamine, red) in cancerous breast tissue. Nuclei counterstained with DAPI (blue). Tissue was fixed in formaldehyde, embedded in paraffin, and 4-5 micron sections cut. Stained sections were viewed using an Olympus IX70 microscope with a 60x 1.4 NA objective lens, and images acquired with a Photometrics CoolSnap CCD with a 1024X1024 pixel resolution, with a pixel size equivalent to 0.07427 microns in X and Y. Z-stacks were acquired to cover the thickness of the tissue section with a step size of 0.5 microns in the Z-direction, and subjected to deconvolution using SoftWoRx 3.5.1 software. A maximum intensity projection of this stack is included in this image group, Part of this field of view is shown as a projection image in Fig 1A of Meaburn et al., 2009, J Cell Biol 187:801-812.