CIL:134, Caenorhabditis elegans, anchor cell. CIL. Dataset

NCBI Organism:Caenorhabditis elegans; Cell Components:basement membrane

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:134^* Cite

Description

The image shows an anchor cell (red) in the developing female reproductive system of C. elegans pushing through the basement membrane (green) that surrounds it. This type of invasion is also often seen during cancer metastasis.

Technical Details

Anchor cells were labeled with mCherry and laminin with GFP. Images were acquired using a Zeiss AxioImager A1 microscope with a 100X Plan- APOCHROMAT objective and a Zeiss AxioCam MRm CCD camera, controlled by Zeiss Axiovision software (Zeiss Microimaging Inc., Thornwood, NJ). See JW Zeill et al 2009. Nat Cell Biol. 11:183–189.

Biological Sources

NCBI Organism Classification: Caenorhabditis elegans
Cell Type: anchor cell
Cellular Component: basement membrane

Biological Context

Biological Process

Attribution

Names: Eliot Hagedorn
Link: NIGMS BioMedical Beat

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL134
Archival Resource Key (ARK): ark:/b7295/w9cil134

Imaging

Image Type: charge coupled device (CCD)
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: Green fluorescent proteins from Aequorea; mCherryFP

Dimensions

Spatial Axis	Image Size	Pixel Size
X	695px	——
Y	271px	——