This image is part of a group of 4 images supporting the conclusion that hRio2 kinase activity is required for cytoplasmic 18S-E pre-rRNA processing. This image is a control for the experiment and shows the distribution of all 18S rRNA precursors (white), via fluorescence in situ hybridization (FISH) with a 5' ITS1 probe, upon depletion of a protein important in rRNA processing, hRio2,in Hela cells. Depletion of hRio2 leads to the accumulation of 18S-E pre-rRNA in the cytoplasm (white). For the set of four images in this group, FISH pictures were processed in parallel by enhancing levels, followed by setting gamma correction to 0.75.