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Licensing
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13117*  Cite 
Description

High resolution micrograph of cubic membrane array which represents the cell’s method of storing large quantities of lipid and protein bilayers into the smallest possible volume. These membranes can quickly expand into planar sheets when the ampulla or the collecting canal needs to expand. These arrays actually bury the microtubular ribbons between the membrane of the collecting canal and these three-dimensional arrays. TEM taken on 8/8/96 by R. Allen with Zeiss 10A operating at 80kV. Neg. 31,500X. Adapted with permission from the J. Cell Sci. 112:3733-3745, 1999. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Attribution
Names
Richard Allen (University of Hawaii)
Published
J. Cell Sci. 112:3733-3745, 1999
Pubmed
10523509
Link
http://www5.pbrc.hawaii.edu/allen/
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL13117
Archival Resource Key (ARK)
ark:/b7295/w9cil13117
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 3584px 0.63nm
Y 3840px 0.63nm