CIL:13094, Acanthamoeba castellanii, cell by organism, eukaryotic cell, amoeboid cell, Eukaryotic Protist. CIL. Dataset

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13094^* Cite

Description

Time series of cooled CCD fluorescence micrographs of live amoeba syringe-loaded with Rho-N58C-PII. See publication below for details. Live cells were observed with a Zeiss Axiovert 135 fluorescence microscope. Electronic imaging of fluorescent specimens was performed using a Photometrics cooled, charge-coupled device (CCD) camera (PXL-1400) controlled with IPLab Spectrum 3.1P software (Scanolytics, Fairfax, VA) on Macintosh computers. Copyright © 1999 by Company of Biologists and reproduced/adapted with permission in 2011.

Biological Sources

NCBI Organism Classification: Acanthamoeba castellanii
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; amoeboid cell
Cellular Component: lamellipodium; cytoplasm; endocytic vesicle

Biological Context

Biological Process: lamellipodium assembly; endocytosis; regulation of lamellipodium assembly

Attribution

Names: Don Kaiser
Published: D. A. Kaiser, V. K. Vinson, D. B. Murphy & T.D. Pollard, J. Cell Sci. 112, 3779-3790 (1999)
Pubmed: 10523513

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL13094
Archival Resource Key (ARK): ark:/b7295/w9cil13094

Grouping This image is part of a group.

Imaging

Image Type: absorption of illumination
Image Mode: UV/visible/IR photons
Parameters Imaged: UV/visible/IR illumination
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: fluorescent labels; visualization by chemical attribute; syringe loaded Rho-N58C-PII
Processing History: recorded image; intensified CCD (ICCD)
Data Qualifiers: processed data

Sample Preparation

Methods: unfixed tissue
Methods: living tissue; whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	256px	——
Y	176px	——