CIL:12640, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Paramecium multimicronucleatum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:cytoplasm, endosome, clathrin coat; Biological process:cytoplasm organization, endosome organization, clathrin coat assembly;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12640^* Cite

Description

A high resolution image of a clump of at least seven small clathrin cages that are probably connected to an early endosome (below the fracture plane). The morphology of these cages shows a tighter packing than the larger clathrin cages at the parasomal sacs. TEM taken on 7/29/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 31,500X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: cytoplasm; endosome; clathrin coat

Biological Context

Biological Process: cytoplasm organization; endosome organization; clathrin coat assembly

Attribution

Names: Richard Allen (University of Hawaii); C. Schroeder
Link: http://www5.pbrc.hawaii.edu/allen/

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL12640
Archival Resource Key (ARK): ark:/b7295/w9cil12640

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: electron density
Parameters Imaged: elastic scattering of electrons; electron density; elevation
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: transmission electron microscopy (TEM)
Processing History: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: freeze_fracture/freeze_etch
Methods: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3690px	0.63nm
Y	3878px	0.63nm