CIL:12633, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

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NCBI Organism:Paramecium multimicronucleatum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:clathrin coat of endocytic vesicle, early endosome, integral to endosome membrane; Biological process:early endosome to Golgi transport, clathrin coating of Golgi vesicle, plasma membrane to endosome targeting;

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12633^* Cite

Description

A high resolution of a quick-freeze deep-etch rotary shadowed replica of a parasomal sac from which a clathrin-coated preendosomal vesicle has pinched off. The typical clathrin cage structure is preserved. Fractured trichocyst at the lower left. TEM taken on 6/7/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 31,500X. Adapted with permission from the J. Cell Sci. 101:449-461, 1992. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: clathrin coat of endocytic vesicle; early endosome; integral to endosome membrane

Biological Context

Biological Process: early endosome to Golgi transport; clathrin coating of Golgi vesicle, plasma membrane to endosome targeting

Attribution

Names: Richard Allen (University of Hawaii); C. Schroeder
Published: J. Cell Sci. 101:449-461, 1992
Pubmed: 1629255
Link: http://www5.pbrc.hawaii.edu/allen/

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL12633
Archival Resource Key (ARK): ark:/b7295/w9cil12633

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: electron density
Parameters Imaged: elastic scattering of electrons; electron density; elevation
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: transmission electron microscopy (TEM)
Processing History: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: freeze_fracture/freeze_etch
Methods: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3732px	0.63nm
Y	4192px	0.63nm