CIL:12627, Mus musculus. CIL. Dataset

NCBI Organism:Mus musculus; Biological process:oocyte maturation

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12627^* Cite

Description

Fluorescent image of a mouse ovary labeled with an antibody to vasa (involved in oocyte differentiation and polarity) which is localized in the oocytes within the ovary. The sample was fixed in paraformaldehyde for subsequent immunolabeling with an antibody to vasa and a secondly antibody conjugated to Alexa 488. The image was captured with a 4MP RGB/gray SPOT digital camera mounted on a Leica MZII Pursuit fluorescent stereoscopic microscope.

Biological Sources

NCBI Organism Classification: Mus musculus
Cell Line: Ovary
Cellular Component: vasa

Biological Context

Biological Process: oocyte maturation

Attribution

Names: Prue Talbot

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL12627
Archival Resource Key (ARK): ark:/b7295/w9cil12627

Imaging

Image Type: recorded image
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: Alexa Fluor 488; primary antibody plus labeled secondary antibody
Processing History: unprocessed raw data

Sample Preparation

Methods: formaldehyde fixed tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	800px	——
Y	600px	——