CIL:12624, Mesocricetus auratus, oocyte. CIL. Dataset

NCBI Organism:Mesocricetus auratus; Cell Types:oocyte

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12624^* Cite

Description

Surface of the zona pellucida surrounding a hamster oocyte. This scanning electron micrograph shows the zona pellicida around a hamster oocyte at high magnification. The zona pellicida has a complex weave and is comprised of three glycoproteins, ZP1, ZP2 and ZP3. The sample was fixed using glutaraldehyde and osmium tetroxide, dehydrated in ethanol, critically point dried, coated with gold, and examined in a Phillips XL30 FEG scanning electron microscope. Magnification: 5851x.

Biological Sources

NCBI Organism Classification: Mesocricetus auratus
Cell Type: oocyte
Cellular Component: zona pellucida

Attribution

Names: Prue Talbot

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL12624
Archival Resource Key (ARK): ark:/b7295/w9cil12624

Imaging

Image Type: recorded image
Image Mode: scanning electron microscopy (SEM)
Parameters Imaged: elastic scattering of electrons
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: gold
Processing History: unprocessed raw data
Data Qualifiers: raw, unprocessed data

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; critical_point dried specimen
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	619px	0.035µm
Y	484px	0.035µm