CIL:12401, Leishmania mexicana, Mus musculus, macrophage, protozoan parasites. CIL. Dataset

NCBI Organism:Leishmania mexicana, Mus musculus, ; Cell Types:, macrophage; Biological process:, ;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12401^* Cite

Description

Leishmania mexicana amastigotes in their parasitophorous vacuole in a mouse macrophage. Murine macrophages (cell line J774) were infected in vitro with Leishmania mexicana stationary phase promastigotes (WHO strain MNYC/BZ/62/M379). Four days post infection the cells were fixed with glutaraldehyde, post-fixed with osmium tetroxide, dehydrated in ethanol and embedded in epoxy resin. Thin sections (~90 nm) were viewed on a Philips Technai 12 transmission electron microscope operating at 80 kV. Magnification 2900X.

Biological Sources

NCBI Organism Classification: Leishmania mexicana; Mus musculus
Cell Type: protozoan parasites; macrophage
Cell Line: WHO strain MNYC/BZ/62/M379; J774
Cellular Component: parasite-containing vacuole

Biological Context

Biological Process: amastigote form Leishmania mexicana; parasite entry into host cell forming a parasitophorous vacuole

Attribution

Names: Eva Gluenz

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL12401
Archival Resource Key (ARK): ark:/b7295/w9cil12401

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: osmium tetroxide
Processing History: unprocessed raw data
Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation To Intact Cell: sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	5112px	3.28nm
Y	4576px	3.28nm