CIL:12370, Nephrotoma, Nephrotoma suturalis, primary spermatocyte. CIL. Dataset

NCBI Organism:Nephrotoma, , ; Cell Types:primary spermatocyte Cell Components:spindle, nuclear chromosome; Biological process:male meiosis chromosome segregation

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12370^* Cite

Description

Time series images showing the dynamics of kinetchore microtubules during meiosis I in primary spermatocytes of the crane-fly Nephrotoma suturalis that were experimentally flattened. Time-lapse polarization microscopy using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy (LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 2.0x zoom lens. Images captured every 15 sec using a QImaging Retigo EXi CCD camera. Raw images were processed using 5-frame algorithm (Shribak and Oldenbourg, 2003). A movie of the time series is included in this grouped set.

Biological Sources

NCBI Organism Classification: Nephrotoma; Nephrotoma suturalis
Cell Type: primary spermatocyte
Cellular Component: spindle; nuclear chromosome

Biological Context

Biological Process: male meiosis chromosome segregation

Attribution

Names: James R. LaFountain; Rudolf Oldenbourg
Published: LaFountain JR,et al. (2004) Mol Cell Biol 15, 5724-5732.
Published: Oldenbourg R, Mei G (1995) J Microsc 180, 140-147
Published: Shriback M, Oldenbourg R (2003) Appl Opt 42, 3009-3017
Pubmed: 15469981
Pubmed: 8537959
Pubmed: 12790452

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL12370
Archival Resource Key (ARK): ark:/b7295/w9cil12370

Grouping This image is part of a group.

Imaging

Image Type: charge coupled device (CCD); Processed image based on 5 recorded images
Image Mode: polarization microscopy
Parameters Imaged: UV/visible/IR photons
Source of Contrast: intrinsic birefringence
Visualization Methods: relative retardation (retardance) measurement
Processing History: 5-frame algorithm

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	678px	87nm
Y	518px	87nm

Time	15 seconds	60