CIL:12337, Euplotes sp., cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Euplotes sp.; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:oral apparatus, cytoplasm; Biological process:oral apparatus organization, cytoplasm organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12337^* Cite

Description

The transition from 3 to 2 rows of cilia can be seen in this view of the AZM. TEM taken on 7/24/67 by R. Allen with Philips 200 operating at 60kV. Neg. 1,370X. Bar = 5µm. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol, and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image is available in the library at (CIL:38890) for qualitative analysis. Additional information is available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Euplotes sp.
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Euplotes sp. from Carolina Biological Supply
Cellular Component: oral apparatus; cytoplasm

Biological Context

Biological Process: oral apparatus organization; cytoplasm organization

Attribution

Names: Richard Allen
Link: http://www5.pbrc.hawaii.edu/allen/

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL12337
Archival Resource Key (ARK): ark:/b7295/w9cil12337

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; Negative scanned for Photoshop.
Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1387px	——
Y	2055px	——