CIL:12329, Mus musculus, fibroblast. CIL. Dataset

NCBI Organism:Mus musculus; Cell Types:fibroblast Cell Components:actin cytoskeleton, nucleus, ;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12329^* Cite

Description

NIH3T3 cells showing the localization of nuclear transport factor RanBP1 and F-actin in interphase cells. Cells were fixed in formaldehyde and labeled with a primary antibody to RanBP1 and Cy3-conjugated secondary to (RanBP1), FITC-labelled phalloidin, and DAPI. Images were collected on a Zeiss LSM510 confocal using a scan speed 6, 8-line averaging and a 63X 1.4NA objective.

Biological Sources

NCBI Organism Classification: Mus musculus
Cell Type: fibroblast
Cell Line: NIH/3T3
Cellular Component: actin cytoskeleton; nucleus; nuclear transport factor RanBP1

Attribution

Names: Hicham Mahboubi; Ursula Stochaj

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL12329
Archival Resource Key (ARK): ark:/b7295/w9cil12329

Imaging

Image Type: recorded image
Image Mode: single-spot confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: 4',6-diamidino-2-phenylindole (DAPI); Fluorescein (FITC); phalloidin; Cy3; primary antibody plus labeled secondary antibody
Processing History: color merge
Data Qualifiers: raw, unprocessed data

Sample Preparation

Methods: formaldehyde fixed tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1024px	0.8µm
Y	1024px	0.8µm

Channel	Wavelength
1	DAPI, FITC, CY3nm