CIL:12327, Halteria grandinella, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Halteria grandinella; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:macronucleus, oral apparatus, food vacuole; Biological process:macronucleus organization, oral apparatus organization, regulation of response to food;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12327^* Cite

Description

This group represents a serial series of sections. At the level of this section, the bottom of cytopharyngeal tube with one cilium remaining. End of macronucleus, three membranelles of the pek, and many food vacuoles are seen. TEM taken on 3/12/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 3,800X. Bar = 2µm. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared thicker to accommodate the series. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Halteria grandinella
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Carolina Biological Supply Co., NC, U.S.A.
Cellular Component: macronucleus; oral apparatus; food vacuole

Biological Context

Biological Process: macronucleus organization; oral apparatus organization; regulation of response to food

Attribution

Names: Richard Allen
Link: http://www5.pbrc.hawaii.edu/allen/

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL12327
Archival Resource Key (ARK): ark:/b7295/w9cil12327

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; Print scanned for Photoshop
Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1612px	——
Y	1577px	——